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        1. 產品詳情
          • 產品名稱:a2PI ELISA Kit

          • 產品型號:
          • 產品廠商:國內供應1
          • 產品文檔:
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          簡單介紹:
          a2PI ELISA Kit
          詳情介紹:
          Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of a2PI in bovine serum, plasma, tissue homogenates and other biological fluids.
          Sample Type Biological Fluids, Plasma, Serum, Tissue Homogenate
          Analytical Method Quantitative
          Detection Method Colorimetric
          Specificity This assay has high sensitivity and excellent specificity for detection of this index.
          Sensitivity 7.7 ng/mL
          Characteristics Bovine Alpha-2-Plasmin Inhibitor (a2PI) ELISA Kit
          Components
          • Pre-coated, ready to use 96-well strip plate
          • Standard (freeze dried)
          • Standard Diluent
          • Detection Reagent A
          • Detection Reagent B
          • Assay Diluent A
          • Assay Diluent B
          • TMB
          • Stop Solution
          • Wash Buffer (30X)
          • Plate sealer for 96 wells
          • Instruction manual
          Material not included
          1. Microplate reader with 450 ± 10nm filter.
          2. Precision single or multi-channel pipettes and disposable tips.
          3. Eppendorf Tubes for diluting samples.
          4. Deionized or distilled water.
          5. Absorbent paper for blotting the microtiter plate.
          6. Container for Wash Solution.
          Gene ID 282522
          UniProt P28800
          Assay Time 1 - 4.5 h
          Plate Pre-coated
          Assay Procedure

          The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

          Assay Precision
          • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
          • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
          • CV(%) = SD/meanX100
          • Intra-assay: CV<10%
          • Inter-assay: CV<12%
          Restrictions For Research Use only
          Handling Advice The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
          Storage 4 °C,-20 °C
          Expiry Date 12 months
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